Skip to main content
. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: Brain Res. 2015 Sep 30;1638(Pt A):42–56. doi: 10.1016/j.brainres.2015.09.020

Figure 3. A representation of a potential genome editing method to create multiple isogenic HD iPSC lines.

Figure 3

CRISPR technology can target and modify specific genes by inducing DNA double strand breaks which triggers the cells DNA-repair machinery to correct the break via homologous recombination. With CRISPR and HTT specific gRNAs, introduction of a donor template DNA with varying lengths of CAG repeats allows generation of isogenic iPSC lines, which differ only in CAG repeat length in exon 1 the HTT gene.