Table 1.
Characterisation of monoclonal antibodies. The antibodies were tested by ELISA using OMPs from both CDM-Fe and CDM+Fe grown bacteria. The antibody which shows 1.0 or more than 1.O OD value with CDM-Fe OMPs were selected as an antibody against IROMPs of A. baumannii and used for subsequent experiments.
| Monoclonal | ELISA | Ig Isotype | Titre in ELISA | |
| Antibodies | Fe- | Fe+ | ||
| 2G9ABIR | 2.762 | .141 | IgM | 1:100 |
| 3D5ABIR | 2.722 | .135 | IgM | 1:3200 |
| 5D6ABIR | 3.093 | .562 | IgM | 1:100 |
| 1D11ABIR | 2.925 | .615 | IgM | 1:8000 |
| 1F7ABIR | 2.975 | .412 | IgM | 1:800 |
| Preimmune sera | .452 | .458 | - | 1:200 |