Table 1.
Enzyme activity of recombinant PlUGT2.
| Specific activitiesa | Relative | |
|---|---|---|
| (nmol mg protein-1 mi-1) | activity (%)b | |
| 4′-O-glucosylation activity | ||
| Genistin | 0.15 ± 0.07 | 0.11 ± 0.05 |
| Daidzin | 0.49 ± 0.06 | 0.36 ± 0.05 |
| Purearin | 0.19 ± 0.07 | 0.14 ± 0.05 |
| Neoliquiritinc | 0.87 ± 0.21 | 0.63 ± 0.15 |
| 7-O-glucosylation activity | ||
| Sophoricoside | 2.62 ± 0.16 | 1.91 ± 0.11 |
| Formononetin | 114.33 ± 4.35 | 83.48 ± 3.17 |
| Liquiritinc | 0.12 ± 0.01 | 0.08 ± 0.01 |
| 4′ 7-O-diglucosylation activity | ||
| Genistein | 136.96 ± 4.24 | 100.00 ± 3.09 |
| Daidzein | 53.32 ± 1.95 | 38.93 ± 1.43 |
| Naringeninc | 7.66 ± 1.32 | 5.59 ± 0.97 |
| Liquiritigeninc | 16.20 ± 3.72 | 11.83 ± 2.71 |
| 3′,4′,7-O-tri-glycusylation activity | ||
| 3′,4′,7-trihydroxyisoflavone | 29.80 ± 2.94 | 21.76 ± 2.15 |
aThe specific activities were measured based on the initial velocity, and the values were calculated by the average of three replicates of the reactions. bThe relative activity toward genistein was set as 100, and that toward other substrates was normalized accordingly. cIndicates that the acceptors are flavanones.