Table 2.

Anti-tumor investigations of extracts and saponins isolated from Polygonum hydropiper.

Samples	Concentrations (μg/ml)	Average inhibition	Percent inhibition	LC50 (μg/ml)
Ph.Cr	1000	21.66 ± 0.66	72.20 ± 2.2***	342.53
	500	17.66 ± 0.33	58.86 ± 1.1***
	250	13.00 ± 1.15	43.33 ± 3.8***
	125	09.33 ± 0.88	31.10 ± 2.9***
	62.5	09.33 ± 0.33	31.10 ± 1.1***
	31.25	06.00 ± 0.57	20.00 ± 1.9***
Ph.Hex	1000	17.00 ± 0.00	56.66 ± 0.0***	657.72
	500	14.33 ± 0.88	47.76 ± 2.9***
	250	11.33 ± 0.33	37.76 ± 1.1***
	125	08.66 ± 0.88	28.86 ± 2.9***
	62.5	07.00 ± 1.73	23.33 ± 5.7***
	31.25	05.33 ± 1.45	17.76 ± 4.8***
Ph.Chf	1000	25.66 ± 0.66	85.53 ± 2.2*	73.81
	500	21.33 ± 0.88	71.10 ± 2.9***
	250	17.00 ± 0.57	56.66 ± 1.9***
	125	16.66 ± 0.00	55.53 ± 0.0***
	62.50	14.33 ± 0.88	47.76 ± 2.9***
	31.25	14.33 ± 0.33	47.76 ± 1.1***
Ph.EtAc	1000	24.00 ± 0.57	80.00 ± 1.9**	217.19
	500	19.33 ± 0.33	64.43 ± 1.1***
	250	15.66 ± 0.33	52.20 ± 1.1***
	125	13.00 ± 1.15	44.33 ± 3.8***
	62.50	11.66 ± 0.66	38.66 ± 2.2***
	31.25	10.00 ± 0.00	33.33 ± 0.0***
Ph.Bt	1000	20.00 ± 0.57	66.66 ± 1.9***	439.83
	500	16.00 ± 0.57	53.33 ± 1.9***
	250	11.33 ± 0.88	37.76 ± 2.9***
	125	10.00 ± 0.57	33.33 ± 1.9***
	62.50	07.66 ± 0.33	25.53 ± 1.1***
	31.25	06.00 ± 0.57	20.00 ± 1.9***
Ph.Aq	1000	16.66 ± 0.66	55.53 ± 2.2***	881.12
	500	11.00 ± 0.00	36.66 ± 0.0***
	250	09.00 ± 1.15	30.00 ± 3.8***
	1250	07.00 ± 1.73	23.33 ± 5.7***
	62.5	06.33 ± 1.45	21.10 ± 4.8***
	31.25	04.33 ± 0.88	14.43 ± 2.9***
Ph.Sp	1000	27.00 ± 0.57	90.00 ± 1.9ns	18.39
	500	24.00 ± 0.00	80.00 ± 0.0ns
	250	22.33 ± 0.33	74.43 ± 1.1*
	125	21.00 ± 1.15	70.00 ± 3.8***
	62.50	18.00 ± 0.57	60.00 ± 1.9***
	31.25	17.33 ± 0.88	57.76 ± 2.9***

Vincristine sulfate was used as positive control having IC₅₀ < 0.1 μg/ml. Values significantly different as compare to positive control,

^*P < 0.05,

^**P < 0.01,

^***:P < 0.001.

ns, Values not significantly different in comparison to standard drug.