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. 2016 Mar 31;7:426. doi: 10.3389/fmicb.2016.00426

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Copyright © 2016 Derakhshani, De Buck, Mortier, Barkema, Krause and Khafipour.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Summary of study design. The experiment was conducted in two replicates (consecutive experimental years) with first 30 and then 20 inoculated calves equally representing all age and dose groups. Overall, 28 out of 50 inoculated calves were identified (indicated by bold numbers) as MAP-positive (based on detection of at least one tissue culture positive, the “gold standard” for identification of MAP infection; Mortier et al., 2013), and along with five control calves were selected for 16S rRNA profiling of the ileal mucosa-associated (MAM) and fecal micoribota.