Table 3.
Structural and subcellular localization analysis of the JcAQPs.
| Name | Len | Mw (KDa) | pI | TMa | Locb | Ar/R selectivity filter | NPA motifs | Froger's positions | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| H2 | H5 | LE1 | LE2 | LB | LE | P1 | P2 | P3 | P4 | P5 | ||||||
| JcPIP1;1 | 287 | 30.73 | 8.60 | 6 | Plas | F | H | T | R | NPA | NPA | E | S | A | F | W |
| JcPIP1;2 | 287 | 30.54 | 8.62 | 6 | Plas | F | H | T | R | NPA | NPA | E | S | A | F | W |
| JcPIP1;3 | 212 | 22.57 | 8.53 | 6 | Plas | F | H | T | R | NPA | NPA | E | S | A | F | W |
| JcPIP1;4 | 286 | 30.46 | 8.72 | 6 | Plas | F | N | A | R | NPA | NPA | Q | S | A | F | W |
| JcPIP2;1 | 284 | 30.35 | 8.20 | 6 | Plas | F | H | T | R | NPA | NPA | Q | S | A | F | W |
| JcPIP2;2 | 286 | 30.60 | 7.62 | 6 | Plas | F | H | T | R | NPA | NPA | Q | S | A | F | W |
| JcPIP2;3 | 285 | 30.33 | 8.67 | 6 | Plas | F | H | T | R | NPA | NPA | Q | S | A | F | W |
| JcPIP2;4 | 280 | 29.91 | 8.97 | 6 | Plas | F | H | T | R | NPA | NPA | M | S | A | F | W |
| JcPIP2;5 | 283 | 29.67 | 9.10 | 6 | Plas | F | H | T | R | NPA | NPA | V | S | A | F | W |
| JcTIP1;1 | 252 | 25.97 | 5.91 | 6 | Plas | H | I | A | V | NPA | NPA | T | S | A | Y | W |
| JcTIP1;2 | 252 | 25.79 | 4.96 | 6 | Vacu | H | I | A | V | NPA | NPA | T | S | A | Y | W |
| JcTIP1;3 | 252 | 25.79 | 5.13 | 6 | Vacu | H | I | A | V | NPA | NPA | T | S | A | Y | W |
| JcTIP1;4 | 254 | 26.39 | 5.83 | 6 | Vacu | H | V | A | V | NPA | NPA | T | S | A | Y | W |
| JcTIP2;1 | 248 | 25.26 | 5.59 | 6 | Vacu | H | I | G | R | NPA | NPA | T | S | A | Y | W |
| JcTIP2;2 | 250 | 25.34 | 5.69 | 6 | Vacu | H | I | G | R | NPA | NPA | T | S | A | Y | W |
| JcTIP3;1 | 257 | 27.34 | 6.49 | 6 | Vacu | H | I | A | R | NPA | NPA | T | A | A | Y | W |
| JcTIP4;1 | 247 | 25.97 | 6.12 | 6 | Vacu | H | I | A | R | NPA | NPA | T | S | A | Y | W |
| JcTIP5;1 | 252 | 26.01 | 7.85 | 6 | Vacu | N | V | G | S | NPA | NPA | I | A | A | Y | W |
| JcNIP1;1 | 275 | 29.27 | 9.21 | 6 | Plas | W | V | A | R | NPA | NPA | F | S | A | Y | L |
| JcNIP2;1 | 291 | 30.80 | 8.85 | 6 | Plas | G | S | G | R | NPA | NPA | L | T | A | Y | I |
| JcNIP3;1 | 277 | 30.04 | 8.50 | 6 | Plas | W | V | A | R | NPA | NPA | F | S | A | F | L |
| JcNIP3;2 | 280 | 30.04 | 5.52 | 6 | Vacu | W | M | A | R | NPA | NPA | F | S | A | Y | I |
| JcNIP4;1 | 263 | 27.82 | 5.89 | 6 | Plas | W | V | A | R | NPA | NPA | F | S | A | Y | I |
| JcNIP5;1 | 298 | 30.85 | 8.87 | 6 | Plas | S | I | A | R | NPA | NPV | F | T | A | Y | L |
| JcNIP6;1 | 307 | 31.37 | 8.71 | 6 | Plas | S | I | A | R | NPS | NPV | L | T | A | Y | L |
| JcNIP7;1 | 265 | 28.17 | 7.00 | 6 | Plas | A | V | G | R | NPA | NPA | Y | S | A | Y | I |
| JcXIP1;1 | 294 | 32.27 | 6.05 | 6 | Plas | I | I | V | R | SPI | NPA | M | C | A | F | W |
| JcXIP2;1 | 303 | 32.29 | 6.59 | 6 | Plas | I | T | V | R | NPV | NPA | L | C | A | F | W |
| JcSIP1;1 | 239 | 25.79 | 9.58 | 6 | Plas | V | L | P | N | NPT | NPA | I | A | A | Y | W |
| JcSIP1;2 | 242 | 26.01 | 9.73 | 6 | Vacu | A | L | P | N | NPT | NPA | M | A | A | Y | W |
| JcSIP1;3 | 243 | 26.00 | 10.02 | 6 | Extr | S | L | P | N | NPT | NPA | M | A | A | Y | W |
| JcSIP2;1 | 241 | 26.37 | 9.57 | 6 | Vacu | S | L | G | S | NPL | NPA | F | V | A | Y | W |
a
Representing the numbers of transmembrane helices predicted by TOPCONS.
b
Best possible subcellular localization prediction by the WoLF PSORT (Chlo, chloroplast; Cyto, cytosol; ER, endoplasmic reticulum; Plas, plasma membrane; Vacu, vacuolar membrane).