Figure 4.

Effect of SWE on the motility of bacteria. (A) SWE effect on S. Enteritidis swimming motility. Swimming motility of S. Enteritidis was determined by adding 200 μl/ml SWE into the agar plates. Single purified colony was inoculated with a toothpick from an overnight TSA plate onto a swim plate (tryptone broth plus 0.3% agar) to observe for effect on motility after overnight incubation at 30°C. (B) SWE effect on S. Enteritidis swarming motility. S. Enteritidis showed deficient movement when inoculated onto swarm plates (Difco bacto-agar, 0.5% glucose) after 24 h incubation at 30°C. (C) Effect of SWE on the relative gene expression of fliD gene required for polymerization of flagellin on the tip of growing flagella. Values represent Mean ± Standard deviation from three independent experiments; each experiment had three biological replicates.