Figure 5.

Effect of SWE treatment on biofilm formation of S. Enteritidis. S. Enteritidis culture was statically grown for 24 h at 37°C in polyvinyl chloride microtitre plates in the presence of 200 μl/ml SWE (SG or CC). (A) Biofilm formation was quantified by staining with crystal violet and determining the optical density at 600 nm. (B) Effect of SWE on the relative gene expression of sdiA gene (homolog of quorum-sensing regulators LuxR). Values with different superscript letters (Tukey multiple mean comparison) are significantly different (one-way Anova; p < 0.05). Values represent Mean ± Standard deviation from three independent experiments; each experiment had three biological replicates. Picture insert: C, positive control; B, negative control; SG200, 200 μl/ml SG extract; CC200, 200 μl/ml CC extract.