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. 2016 Mar 31;7:91. doi: 10.3389/fphys.2016.00091

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Copyright © 2016 Mazelet, Parker, Li, Arner and Ashworth.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Passive force (A), active force (B) and sarcomere length (C) at different lengths (L) relative to slack length (Ls). (A,B) Open circles, 7 dpf larvae held in Embryo Medium; filled circles, 7 dpf larvae held in Embryo Medium after Tricaine immobilization between 17 and 24 hpf; open squares, control siblings of the relaxed strain and filled squares, relaxed immotile mutants (n = 3–8). (C) Control siblings (Rr, dark gray), mutants (rr, light gray), and Tricaine-BTS treated larvae (BTS, mid gray). We observed that at stretches above 60% the controls siblings tended to break. The Tricaine-BTS and relaxed mutant larvae were possible to stretch significantly further without breaking, up to 80%. However, the sarcomere length did not increase further above 70%.