Figure 6.

Small angle X-ray diffraction. Relaxed immotile mutants (D, Filled circles), their control siblings (C, open circles) at 6 dpf, larvae treated with Tricaine between 17 and 24 hpf and recovered until 6 dpf (B, filled squares) and their controls kept in Embryo Medium until 6 dpf (A, open squares). Panels (F,G) shows the spacing (d10, d11) of the 1.0 and 1.1 reflections at different degrees of stretch, (H) shows the relationship between d1.0² and inverted value of stretch. Panel (I) shows the intensity ratio of the 1.1 and 1.0 reflection at different degrees of stretch. n = 4–6 except in the controls kept in Embryo Medium until 6 dpf, where n = 1. Panel (E) shows a relaxed immotile mutant sample in rigor, displaying an increase in the outer 1.1 reflection, the 1.1/1.0 intensity ratio was 3.67 compared to about 0.4 in samples under normal conditions (D,I).