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. 2016 Mar 31;10:126. doi: 10.3389/fnins.2016.00126

Figure 2.

Figure 2

Structure of the parietal cortex on P5 and P20 in control rats and rats subjected to prenatal hypoxia on E14 or E18. (A) Double labeling of cortical tissue in a control rat on P5 with EdU labeling performed on E14. EdU-positive cell nuclei were stained by AlexaFluor488, Fox3-positive neurons stained by PE-conjugated secondary antibodies. Scale bar 50 μm. (B) Position of the analyzed areas in the parietal cortex (Bregma +0.20 mm; Paxinos and Watson, 2006). (C) Double labeling of cortical tissue in a control rat on P20 with EdU labeling performed on E14. EdU-positive cell nuclei were stained by AlexaFluor488, Fox3-positive neurons stained by PE-conjugated secondary antibodies. Scale bar 50 μm. (D) A coronal section of the cortical tissue of a control rat on P5, Nissl staining. Scale bar 200 μm. (E) A coronal section of the cortical tissue of a control rat on P5. EdU labeling (indicated by an arrow) on E14. The majority of the cells labeled on E14 are placed in the lower (V-VI) cortical layers. (F) A coronal section of the cortical tissue of a rat, exposed to hypoxia on E14, on P5. EdU labeling was performed on E14. (G) A coronal section of the cortical tissue of a control rat on P5 with EdU labeling performed on E18. The majority of the cells labeled on E18 are placed in the superficial cortical layers II-III. (H) A coronal section of the cortical tissue of a rat, exposed to hypoxia on E18, on P5. EdU labeling was performed on E18. (I) A coronal section of the cortical tissue of a control rat on P20, Nissl staining. Scale bar 200 μm. (J) A coronal section of the cortical tissue of a control rat on P20. EdU labeling performed on E14.