Table 3.
Type of cells | Days after birth | ||||
---|---|---|---|---|---|
P10 | P20 | P30 | P60 | P90 | |
The number of cortical cells in rats exposed to hypoxia on E18 in % to control | |||||
Small non-pyramidal | 97.1 ± 12.6 | 110.4 ± 11.2 | 100.0 ± 11.5 | 92.0 ± 10.1 | 115.8 ± 21.1 |
Big non-pyramidal | 95.0 ± 15.3 | 95.7 ± 10.5 | 115.6 ± 8.8 | 94.5 ± 7.8 | 99.7 ± 17.4 |
Small pyramidal | 104.3 ± 5.8 | 101.4 ± 8.2 | 92.4 ± 15.2 | 115.1 ± 19.9 | 92.8 ± 15.2 |
Big pyramidal | 102.8 ± 3.7 | 110.6 ± 11.6 | 108.4 ± 7.2 | 100.0 ± 4.4 | 97.9 ± 15.5 |
Total cell number | 100.5 ± 4.4 | 99.1 ± 3.8 | 90.7 ± 9.8 | 105.2 ± 9.4 | 105.0 ± 9.2 |
GFAP-positive cells | 101.7 ± 3.2 | 104.6 ± 4.8 | 105.2 ± 6.7 | 104 ± 6.4 | 94.7 ± 8.9 |
Fox3 (NeuN)-positive | 96.8 ± 8.4 | 108.5 ± 9.6 | 91.4 ± 11.5 | 93.5 ± 7.7 | 94.1 ± 10.5 |
Neural cells were classified by their size (area) and shape (ratio of the longest to the shortest axes) of the cell body. Data are presented as the ratio (mean number of cells in rats exposed to hypoxia/mean number of cells in control rats) in % ± Standard error of the difference between the means of two samples. 100%—cell number in control rats. In each group of animals (n = 8) 10 cortical tissue areas of each animal were analyzed by Nissl staining or immunohistochemistry. No changes (compared to control) in rats, exposed to hypoxia on E18, were observed.