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. 2016 Mar 31;10:13. doi: 10.3389/fncir.2016.00013

Figure 5.

Figure 5

Twenty-four hours of water deprivation induced strong Fos expression in LHbMC; most of the activated neurons were GABA immunopositive. (A). WD24 produced a spatial pattern of neuronal activation which coincided with AVP+ fiber distribution, i. e. the medial central nucleus of the lateral habenula LHbMC. Inset shows Gad2 in situ hybridization pattern in LHbMC (obtained from the Allen Institute for Brain Science (http://mouse.brain-map.org/experiment/show/79591669). Panels (B–D) confocal images for coronal sections of habenula with double immunofluorescence showing that most of the Fos+ cells were GABA+ neurons. White consecutive numbers and yellow arrows indicate Fos+/GABA+ neurons, whereas blue consecutive numbers indicate Fos+/GABA- neurons. Panels (Da-b) show the magnified squared region of (B), where 6 Fos+ nuclei are found, 4 of them had GABA+ cytoplasms (yellow arrows) and 2 had GABA negative cytoplasms (red arrows). Scale bar: 50 μm.