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. 2016 Mar 31;10:13. doi: 10.3389/fncir.2016.00013

Figure 7.

Figure 7

In vivo juxtacellular labeled individual LHbMC cells confirmed the interneuron identity of GABA cells. (A) : a camera lucida reconstruction of a juxtacellularly labeled cells superimposing 8 coronal sections of 70 μm, respectively. (Aa) The soma was located in the lateral part of the LHbMC, around Bregma (Br) −3.84 mm, according to Rat Brain Atlas (Paxinos and Watson, 2006). (Ab) Labeled axons were concentrated around the rostro-caudal coordinate Br –3.48 mm. (Ac) Is amplification of (Ab) squared region. (Ae) and (Ad): The soma and axon terminals were tested immunopositive to GABA and Gad 65/67, respectively. (Af): A close contacting relationship was observed between the neurobiotin labeled dendrite and VP+ axons. (B): Another example of in vivo juxtacellularly labeled GABAergic interneuron. Camera lucida reconstruction was obtained from 10 coronal sections of 70 μm, respectively. (Ba) Soma (B) was located in the lateral part of the LHbMC, around Br −3.72 mm. (Bb) The main axonal ramification was found in sections caudal to the soma. (Bc) is an amplification of the squared region of (Bb). The axon was tested to be GAD65/67+ with confocal microscopy (Bd) while the soma was GABA+ (Be). This cell's dendrites were also contacting AVP+ axons (Bf). Scale bars: 20 μm.