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. 2016 Mar 31;10:85. doi: 10.3389/fncel.2016.00085

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Copyright © 2016 Huang, Yang, Chen, Xing, Guo, Zhu, Li, Li, Zhang and Feng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The GAP domain is required for the porf-2's function on NSC proliferation. (A) The construct pattern of the domain deleted plasmid. Verification of domain deleted plasmid by immunoblot. The same blot was probed with β-actin antibody as a loading control. (B) The morphology of neurosphere in different groups. Scale bar: 50 μm. (C) Quantification of neurosphere size in each group. ^*p < 0.05, ^**p < 0.01. (D) Representative images of Edu positive cells in indicated group. Quantification of the percentage of Edu positive cell number in each group. Scale bar: 50 μm. Data are mean ± SEM (n = 5). ^**P < 0.01.