Skip to main content
. 2016 Mar 31;10:85. doi: 10.3389/fncel.2016.00085

Figure 5.

Figure 5

Porf-2 inhibits NSC proliferation through wnt/β-catenin pathway. (A) The expression and quantification of p-PTEN, PTEN, p-PI3K, PI3K, p-Akt, Akt, p-mTOR, mTOR, NICD, and β-catenin protein in control and porf-2 overexpression group. Data are mean ± SEM (n = 3). β-actin was used as a loading control. (B) The expression and quantification of β-catenin protein in cytoplasm and nucleus in control and porf-2 overexpression group. GAPDH and HistoneH3 were used as loading controls for protein in cytoplasm and nucleus respectively. **p < 0.01. (C) Confocal microscopy image of β-catenin expression in NSCs after lentivirus infection. White arrow indicated the expression of β-catenin in nucleus in control group. Blue arrow indicated the expression of β-catenin in nucleus in porf-2 overexpression group. Scale bar: 50 μm. (D) The expression and quantification of β-catenin protein in cytoplasm and nucleus in different groups. Data are mean ± SEM (n = 3). **p < 0.01.