Figure 1.

Protein recovery yield in function of precipitation methods, collection times, and confluence levels measured by the Bradford assay. The yield of protein recovered following the two tested protein precipitation methods (TCA-DOC and TCA-NLS-THF) and four different culture conditions was measured by the Bradford assay. The amount of recovered protein for all the tested conditions is given in μg/ml of conditioned-supernatant. The TCA-DOC method consistently gave better recovery yields independent of the induction conditions that were used. ^***Corresponds to a P-value of < 0.0001.