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. 2016 Mar 31;10:70. doi: 10.3389/fncel.2016.00070

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Copyright © 2016 Paré, Deschênes, Pouliot, Dupré and Gros-Louis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Visualization and quantification of the protein spots of different tested induction conditions and precipitation methods. Proteins were conjugated with the different CyDyes after extraction with the TCA-DOC, 24 h, post-confluence (Cy2) (A), TCA-NLS-THF, 48 h, post-confluence (Cy3) (B) and TCA-DOC, 48 h post-confluence (Cy5) (C) induction conditions and precipitation methods and resolved by 2D-DIGE from one control cell line. Each individual spot is circled and was automatically quantified by the software (far right). Note that the TCA-DOC 48 h post-confluence induction condition, which was also consistently given better protein recovery yield, gave the highest number of protein spots.