Table 3.
Detailed migration protocol for the 2D gel electrophoresis.
| U (V) | Time or Vh | μA (I/strip) | Temperature (°C) | |
|---|---|---|---|---|
| Step 1 | 500 | 500 | 75 | 20 |
| Step 2 | 1000 | 800 | 75 | 20 |
| Step 3 | 10,000 | 16,500 | 75 | 20 |
| Step 4 | 10,000 | 32,200 | 75 | 20 |
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