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. 2016 Mar 31;7:120. doi: 10.3389/fphys.2016.00120

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Copyright © 2016 El Mourabit, Loeuillard, Lemoinne, Cadoret and Housset.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Time course of cell markers in PMF culture. PMF culture was subjected at different time points, to (A) RT-PCR analysis of cytokeratin 19 (CK19), ecto-nucleoside triphosphate diphosphohydrolase 2 (Entpd2), Vimentin, Acta2/α-SMA, Col1a1, Col15a1, Fibulin 2, and CD90/Thy-1; mRNA levels are normalized for Hprt and reported relative to those measured immediately after bile duct isolation (D0). They represent the means ± standard error of 2–3 preparations. (B) Immunofluorescence of cytokeratin (CK), and of α-SMA co-labeled with CK or COL15A1; nuclei were stained with DAPI; z images at 5.7 (Z04) and 13.6 (Z27) μm from the substrate are shown. D, days; Scale bars, 50 μm.