Table 4.

Potential (ζ) and Dynamic Light Scattering (DLS).

Reducing agent	1 month	1 month		2 months
	ζ Potential (mV)	RH (nm)	PDI	RH (nm)	PDI
1. Native HSA pH 6	−9 mV (±1)	25.6 nm	0.54	29.8 nm	0.51
2. NEM-treated HSA pH 6a	−	24.9 nm	0.56	27.2 nm	0.46
3. Heat-denaturated HSA pH 6b	−	−	−	−	−
4. Native HSA pH 7	−11.7 mV (±1)	31.2 nm	0.48	24.1 nm	0.46
5. NEM-treated HSA pH 7a	−	34.3 nm	0.47	27.4 nm	0.50
6. Heat-treated HSA pH 7	−27 mV (±1)	31.6 nm	0.37	27 nm	0.42
7. Native BSA pH 6	−4.5 mV (±1)	24.2 nm	0.55	32.9 nm	0.46
8. NEM-treated BSA pH 6	−5 mV (±1)	24.6 nm	0.54	47.2 nm	0.49
9. Heat-treated BSA pH 6a	−	27.4 nm	0.54	35.9 nm	0.50
10. Native BSA pH 7	−17 mV (±2)	35.8 nm	0.48	28.3 nm	0.40
11. NEM-treated BSA pH 7a	−	34.7 nm	0.46	28.2 nm	0.42
12. Heat-denaturated BSA pH 7	−8 mV (±1)	35.4 nm	0.47	25.8 nm	0.39

^aζPotential values did not stabilize.

^bMeasurements impaired by precipitation of the GNPs.