Figure 3.

Altered morphology of neurites in the PrL of adult female mice born to dams that were administered NaAsO₂ during gestation. (A) Representative photomicrographs of the PrL in Golgi- and Cresyl Fast Violet-stained coronal brain sections from female mice with or without prenatal exposure to NaAsO₂. The rectangular area was magnified and shown in the corresponding right panel. Bars = 20 μm (low magnification) and 10 μm (high magnification). (B) Demonstrative photomicrograph for the intersection points (indicated by red arrows) between a virtual sphere “Space Ball” (white circle) and Gogli-stained neurite. (C) Estimated neurite lengths in Golgi-stained PrL neurons as measured by the Space Ball method. Values (mean ± SEM) were normalized to the estimated volume of the selected PrL region (control group n = 7, NaAsO₂-exposed group n = 5). An asterisk indicates p < 0.05 vs. the control group.