Figure 6. Maximum likelihood (ML) tree based on aligned amino acid sequences of DNA polymerase type-B family catalytic domain (POLBc) superfamily with 2,000 ML bootstrap replications.

A public POLBc database was used from the conserved domain database (CDD) (Supplementary Table S8). Each cluster indicates a subfamily of POLBc superfamily. The novel POLBc subfamily clade comprises the POLBc-related partial domains of the hypothetical gene in this study. Eight public domain data are not identified to a specific subfamily in the POLBc superfamily (bright orange; GI: 16081956, 48477200, 15789745, 124362982, 48425269, 48425257, 6435552, 147921691). The DNA polymerase type-II B (Pol II B; bright green) subfamily is not monophyletic. Most subfamilies of POLBc are monophyletic; however, some inter-clade relationships differ slightly from the public POLBc superfamily database (CDD cl00145).