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. 2016 Mar 31;6:23689. doi: 10.1038/srep23689

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Copyright © 2016, Macmillan Publishers Limited

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(A) A single nucleotide polymorphism assay was developed to detect the presence of glutamic acid or glutamine at position 35 of KIR2DL2/L3 alleles. (B) Representative CD107 degranulation assays in which single KIR2DL2/L3⁺ NK cells were tested for missing-self recognition using 721.221 cells without C1 ligand. (C) Summary results of CD107 degranulation assay from 4–5 donors with each allelic group. (D) CD107 inhibition by different KIR2DL2/L3 allelic groups when stimulated with 721.221 cells expressing Cw07 ligand. Percentage indicates relative value when compared with target cells without Cw07 ligand. Results are average from 4–5 donors in each KIR2DL2/L3 allelic group. P values were calculated using Student T test. Error bars represent SD.