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. 2016 Mar 31;6:23689. doi: 10.1038/srep23689

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Copyright © 2016, Macmillan Publishers Limited

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(A) Stable YT-Indy cell lines expressing KIR2DL2*001 or KIR2DL2*001 mutant with glutamic acid at the amino acid position 35 replaced with glutamine. YT-Indy transfected with empty vector was used as mock control. Similar expression of KIR2DL2/L3 in the cell lines was confirmed by flow cytometry. (B) 721.221 was stably transduced with MSCV vector containing HLA-Cw07 ligand. (C) Specific killing of YT-Indy expressing KIR2DL2*001 or KIR2DL2*001 mutants (2DL2 E³⁵Q) were assessed against 721.221-Cw07 by BADTA release assay. The experiments were repeated at least 3 times. P value was calculated using Student T test. Error bars represent SD.