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. 2016 Mar 31;6:23670. doi: 10.1038/srep23670

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Copyright © 2016, Macmillan Publishers Limited

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SF2 cells transfected with no plasmid (control), mock expression plasmid, wild-type GDF5, or mutant GDF5 for 0, 5, 15, 30, or 60 min were analysed by Western blot using anti-Smad5 or anti-phospho Smad1/5/8 antibodies (a). Similarly, cells transfected with no plasmid (control), mock expression plasmid, wild type GDF5, or mutant GDF5 were also stimulated for 30 min with BMP2, and the expression of Smad5 and phospho-Smad1/5/8 was evaluated (b).