Figure 1. Workflow used to nanomanufacture EBOV-TMV nanoparticles.

From left to right: Nicotiana benthamiana plants can be used to produce TMV particles. The structure of TMV is shown highlighting the protein in green and its RNA in orange (the image was created using 2TMV PDB file and the UCSF chimera software [http://www.cgl.ucsf.edu/chimera]). Extracted TMV is dissembled followed by reassembly using purified TMV coat proteins (CPs) and synthetic RNA transcripts to yield the Ebola-TMV mimic (EBOV-TMV). A schematic of the RNA transcript design is shown above (for detailed information see Supplementary Information).