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. 2016 Mar 31;6:23795. doi: 10.1038/srep23795

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Copyright © 2016, Macmillan Publishers Limited

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(A–C) Bright field photomicrograph shows brownish PLIN⁺ LLC expressing TNF-α mRNA (blue) in the cerebral cortex. In situ hybridization for TNF-α mRNA, positively detected using alkaline phosphatase (arrowheads indicate blue staining), was done in combination with immunoperoxidase for PLIN (arrows indicate brown staining) in old brain tissue. Scale bars: 20 μm.