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. 2016 Mar 31;6:23795. doi: 10.1038/srep23795

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Copyright © 2016, Macmillan Publishers Limited

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(A) PLIN⁺ Iba-1⁺ microglial cells. (B) PLIN⁺ NeuN⁺ neurons. (C) PLIN⁺ GFAP⁺ glia limitans astrocytes. (D) PLIN⁺ cells adjacent to α-SMA⁺ pericytes (white arrowheads) in the brain cortical region. (E) PLIN⁺ S100β⁺ ependymal cells in the medial lateral ventricle wall (white arrowheads). Brain tissue samples were analyzed by immunohistochemistry using the lipid droplet specific marker Perilipin (PLIN, in red) in combination with cellular phenotype markers indicated in the figure (in green). Insets show higher magnification of distinct PLIN⁺ cells. Nuclei were stained with Hoechst (in blue). Bars: 20 μm. Inset bars: 10 μm.