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. 2016 Mar 31;6:23643. doi: 10.1038/srep23643

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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The effect of miR-9041 (A) or miR-9850 (C) overexpression on virus infection in the gills of shrimp. To overexpress the host miRNA, miR-9041-mimic or miR-9850-mimic and WSSV were co-injected into shrimp. At different time post-infection (0, 24, 36, and 48 h), the WSSV copies in shrimp were examined. U6 was used as a control. The influence of miR-9041 (B) or miR-9850 (D) silencing on the virus infection in vivo. AMO-miR-9041 or AMO-miR-9850 and WSSV were co-injected into shrimp. At various time post-infection (0, 24, 36, and 48 h), the WSSV copies in shrimp were evaluated. The numbers showed the time points after WSSV infection in shrimp. Asterisks indicated significant differences (*p < 0.05; **p < 0.01) between treatments.