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. 2016 Mar 31;6:23643. doi: 10.1038/srep23643

Figure 6. The pathway mediated by miR-9041 or miR-9850 in virus infection.

Figure 6

Effects of miR-9041 overexpression on the expressions of Dnm1 (A), Dnm2 (C) or Dnm3 (E) in vivo. Effects of miR-9850 overexpression on the expressions of Dnm1 (G), Dnm2 (I) or Dnm3 (K). miR-9041-mimic or miR-9850-mimic and WSSV were co-injected into shrimp. As a control, WSSV only was included in the injections. At different time after injection (0, 24, 36, and 48 h), the mRNA levels of Dnm1, Dnm2 and Dnm3 were quantified by quantitative real-time PCR. Influence of miR-9041 silencing on the Dnm1 (B), Dnm2 (D) or Dnm3 (F) expressions. Influence of miR-9850 silencing on the Dnm1 (H), Dnm2 (J) or Dnm3 (L) expressions. AMO-miR-9041 or AMO-miR-9850 was injected into WSSV-infected shrimp to knock down the miR-9041 or miRv-9850 expression. WSSV only was used as a control. At different time post-infection (0, 24, 36, and 48 h), the expressions of Dnm1, Dnm2 and Dnm3 were detected with quantitative real-time PCR. Statistically significant differences between treatments were indicated by asterisks (**p < 0.01).