Table 2. The first order rate constants of fluorescence changes during actin assemblyf.
Phase | HMF | GMF | |
---|---|---|---|
Fast phase (0–20 min) | K1H = 8.67 ± 0.99a | K1G = 17.27 ± 2.22**,b,c | K1G′ = 14.28 ± 2.79$,d |
Slow phase (20–60 min) | K2H = 2.84 ± 0.69##,e | K2G = 12.00 ± 6.37 | / |
aData were shown in mean mean ± s.d. from three independent experiments.
bK1H < K1G; **p = 0.0036, calculated by student’s t-test.
cK1G is not significantly different from K2G; p = 0.3932, calculated by paired student’s t-test;
dCalculated from the GMF data from 0 to 60 min (red dashed line); K1H < K1G′; $, p = 0.0224, calculated by student’s t-test.
eK1H > K2H; ##p = 0.0017, calculated by paired student’s t-test.
fThe rate constants (K) are the absolute values in 10−4·s−1.