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. 2016 Mar 23;4(6):e12679. doi: 10.14814/phy2.12679

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© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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CN‐A has an interaction with nephrin. (A) The positive spots were detected with the Duolink technique in a normal rat kidney section incubated with the antibodies for the CN‐A and nephrin, indicating that these molecules located with close proximity, within 40 nm. (B) Double bands of nephrin were detected in whole lysates of the glomeruli, and an upper band was densely detected in the anti‐CN‐A precipitate (arrowhead). ZO‐1 and podocin were also detected in the anti‐CN‐A precipitate. These observations indicated that the CN‐A has an interaction with the SD components (IP, immunoprecipitate; IB, immunoblot; NRS, normal rabbit serum).