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. 2016 Mar 31;4(6):e12708. doi: 10.14814/phy2.12708

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© 2016 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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The influence of aging, obesity, and diet on CD3⁺NK1.1⁻ cells (T cells) and their subpopulations in liver. Liver immune cells were isolated and stained for CD3, NK1.1, CD4, CD8, and CD62L markers and analyzed by flow cytometry. Data are presented as mean ± SE (minimum of five animals/group analyzed individually). Significant differences are indicated (^#compared to YL, *compared to YO, P < 0.05).