Figure 2.
EIPA attenuates CH‐induced migration and proliferation of PASMCs. (A) Representative images showing PASMC migration assessed by transwell assay. Panels show total number of cells on the membrane before and after scraping the top chamber (unmigrated cells) for cells from normoxic (N) and chronically hypoxic (CH) rats. (B) Bar graphs illustrating percent of migrated cells isolated from N and CH rats with (n = 6 for N and n = 4 for CH) and without EIPA (10−5 mol/L; n = 15 for N and n = 5 for CH), a NHE inhibitor. Vehicle treatment had no effect on migration (n = 4). (C) Bar graphs illustrating in vitro proliferation of PASMCs with and without EIPA (10−5 mol/L) isolated from N (n = 23 for untreated and n = 12 for EIPA) and CH (n = 8 each for untreated and EIPA) rats. Vehicle control (n = 4) had no significant effect on proliferation. Proliferation values are presented as percent of N. All bar graphs show mean ± SEM data. *Indicates significant difference from N value (P < 0.05); †indicates significant difference from CH value.