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. 2016 Mar 31;6:23827. doi: 10.1038/srep23827

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(A) Representative Immunohistochemical images of ALP, Osterix and β-catenin (brown, indicated by the arrows). Semi-quantitative analysis showed that icariin treatment obviously increased ALP (B), Osterix (C) and β-catenin (D) positive cells in the calvariae of mice stimulated with Ti particles, while ICG-001 treatment attenuates the effects of icariin. n = 7 per groups. Data are presented as mean ± SD, *p < 0.05, **p < 0.01, one-way ANOVA and Tukey post-hoc pairwise comparisons.