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. 2016 Mar 31;6:23944. doi: 10.1038/srep23944

Figure 5. ChREBP-α increases ChREBP-β transcription by binding to the ChoRE site in the human ChREBP-β promoter.

Figure 5

(a). Luciferase activity analysis for the 2.9 kb, 2.0 kb, 1.0 kb, 0.4 kb and 0.3 kb ChREBP-β promoter fragments in the pGL4-Basic plasmid at 24 hours after the ChREBP-β promoter fragment plasmids and empty vector or HA-ChREBP-α or HA-ChREBP-α and Myc-Mlx expression plasmid are transfected in 293T cells. *indicates p < 0.05 when compared with the other four black bars. (b). DNA sequences of the ChoRE site in the nucleotides −95 to −79 region of the ChREBP-β promoter. (c). Luciferase activity analysis for the 2.9 kb (WT), ΔE-box1, ΔE-box2, and ΔChoRE ChREBP-β promoter in the pGL4-Basic plasmids at 24 hours after the ChREBP-β promoter plasmids and empty vector or HA-ChREBP-α and FLAG-Mlx expression plasmid are transfected in 293T cells. *indicates p < 0.05 when compared with the corresponding empty vector-transfected sample. (d). ChIP analysis for 293T cells transfected with empty vector or FLAG-ChREBP-α and FLAG-Mlx expression plasmid using an anti-FLAG antibody or nonspecific IgG.