Figure 1. High-throughput microfluidic cell culturing and transfection chip.

(a) Schematic of the 280-chamber valve perfusion chip. (b) The different layers of the device were patterned in photoresist. Close-up images show the sieve design (left) and the valve design (right). (c) The chip is loaded in segments of 5 chambers. (d) COMSOL model showing the flow velocity profile of the valve design (Scale: m/s). (e) COMSOL model showing the diffusion of nutrients into the center of the chamber using a 13.4 kDa protein with a diffusion coefficient of 1.14 × 10⁻⁶ cm²/s  as an example. The extent of diffusion (0–1) is indicated.