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. 2016 Mar 31;6:23884. doi: 10.1038/srep23884

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HK2 cells transfected with scrambled siRNA or KCa3.1 siRNA overnight were pretreated with 50 nmol/L Bafilomycin A₁ for 3 hours and then incubated with TGF-β1 (2 ng/ml) for 48 hours. Immunoblot analysis of LC3 with or without KCa3.1 siRNA transfection in TGF-β1-exposed HK2 cells together with Bafilomycin A₁ (a). Quantification of LC3 expression in TGF-β1-treated HK2 cells together with Bafilomycin A₁ (b). Results are presented as mean + SEM. *P < 0.05 and **P < 0.01. N = 4.