Table 1. Nucleotide variation and divergence per chromosomal arrangement for the six genes studied.

Gene	Pop	Arrangement	n	h	S	Singletons	π	πsil	θsil	Ksil
Pif1A	MP	O3+4+1	9	9	104	61	0.021	0.022	0.025	0.188
		O3+4	12	12	120	55	0.021	0.023	0.026	0.191
	BC	O3+4+7	9	9	89	54	0.017	0.018	0.021	0.187
		O3+4	12	12	114	42	0.022	0.023	0.024	0.190
Abi	MP	O3+4+1	3	3	8	8	0.003	0.008	0.008	0.174
		O3+4	12	12	32	20	0.005	0.012	0.017	0.173
	BC	O3+4+7	10	9	33	21	0.006	0.015	0.018	0.172
		O3+4	7	7	24	15	0.006	0.014	0.015	0.173
Sqd	MP	O3+4+1	10	5	12	8	0.002	0.005	0.008	0.115
		O3+4	12	12	25	16	0.005	0.006	0.008	0.116
	BC	O3+4+7	10	10	17	12	0.003	0.004	0.006	0.115
		O3+4	10	10	21	14	0.005	0.006	0.007	0.115
Yrt	MP	O3+4+1	11	11	28	14	0.009	0.031	0.035	0.327
		O3+4	12	12	33	18	0.010	0.034	0.042	0.327
	BC	O3+4+7	9	9	31	18	0.010	0.035	0.013	0.325
		O3+4	12	12	35	21	0.010	0.035	0.041	0.326
Atpα	MP	O3+4+1	9	4	6	6	0.001	0.003	0.005	0.204
		O3+4	12	12	22	9	0.005	0.015	0.013	0.208
	BC	O3+4+7	9	8	22	17	0.004	0.006	0.010	0.204
		O3+4	12	11	27	16	0.005	0.016	0.019	0.209
Fmr1	MP	O3+4+1	10	10	30	24	0.004	0.007	0.010	0.150
		O3+4	11	11	38	25	0.005	0.009	0.012	0.151
	BC	O3+4+7	8	7	19	12	0.003	0.006	0.007	0.150
		O3+4	10	10	28	18	0.004	0.008	0.010	0.151

Pop, population: Mt. Parnes (MP) and Barcelona (BC); n, sample size; h, number of haplotypes; S, number of polymorphic sites; π, nucleotide diversity in all sites; π_sil, nucleotide diversity in synonymous sites and non-coding positions; θ_sil, heterozygosity in silent sites; K_sil, divergence per silent site between D. subobscura and D. pseudoobscura.