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. Author manuscript; available in PMC: 2016 Aug 16.
Published in final edited form as: Sci Signal. 2016 Feb 16;9(415):ra17. doi: 10.1126/scisignal.aac4380

Fig. 3. ONC201-induced apoptosis is independent of the extrinsic pathway of apoptosis.

Fig. 3

(A) Real-time quantitative polymerase chain reaction (PCR) for DR5 and TRAIL mRNA after exposure to ONC201 (5 μM) of Z-138, MINO, JeKo-1, and OCI-AML3 cells. Relative quantity (RQ) values of mRNA expression are calculated as the fold change relative to mRNA expression at time 0 (normalized to GAPDH) (n = 3 experiments). (B) Specific apoptosis of MCL cell lines treated for 72 hours with ONC201 (5 μM), with or without Z-IETD-FMK (25 μM). JeKo-1 cells were also treated with TRAIL (10 nM), with or without Z-IETD-FMK, for 72 hours (n = 3 experiments). (C) Specific apoptosis and number of live cells (PI and annexin V double-negative cells) in parental Jurkat and caspase-8–deficient Jurkat I9.2 cells after treatment with ONC201 (5 μM for 72 hours) (n = 3 experiments). (D) Specific apoptosis induced by ONC201 with or without the pan-caspase inhibitor Z-VAD-FMK (50 μM) in JeKo-1 and Jurkat I9.2 cells (n = 3 experiments). ***P < 0.001, ****P < 0.0001.