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. 2016 Mar 21;5(3):e208. doi: 10.1038/oncsis.2016.17

Figure 1.

Figure 1

High Kaiso expression correlates with shorter metastasis-free survival and EMT. (a) Analysis of the publicly available TCGA breast cancer (BCa) data set revealed that high Kaiso expression correlates with ER (−) negativity and TNBC. **P<0.001. (b) Patients from the TCGA (n=977) and the GEO (GSE20685) (n=327) data sets were segregated into Kaiso (ZBTB33)-high, Kaiso-intermediate and Kaiso-low groups based on transcript levels. Kaplan–Meier survival curves revealed a significant negative correlation between high Kaiso expression, overall survival and distant metastasis-free survival in all BCa cases. Statistical significance was determined by log-rank test and P-values are indicated. (c) RT–PCR and immunoblot analysis of control and Kaiso-depleted MDA-231 and Hs578T cells. (d) Phase-contrast images of control and Kaiso-depleted MDA-231 and Hs578T cells. (e) Phase-contrast images of Kaiso-depleted MDA-231 cells transfected with either an empty or mKaiso vector. Scale bar, 100 μM.