Table 1. Oligonucleotides representing different potential Kaiso-binding sites in the TGFβR1 promoter.
TGFβR1 probe name | Oligonucleotide sequence (5′–3′) | Location |
---|---|---|
KBS-1 WT | CTGATTCCTGCTATCAAGGTTTA | −1212 to −1208 |
KBS-1 MUT | CTGATTCCTTCTATCAAGGTTTA | |
KBS-2 WT | ATTTTGGCGTCGCAGAGGGAAGGTGGGTGGAGCGTCTCGCAGTAAATTAG | −1035 to −1002 |
KBS-2 MUT | ATTTTGGCGTCTCAGAGGGAAGGTGGGTGGAGCGTCTCTCAGTAAATTAG | |
KBS-3 WT | GGTGCTGGGGCTGGCAGACCCCGCC | −289 to −283 |
KBS-3 MUT | GGTGCTGGGGCGGGCAGACCCCGCC | |
KBS-4 WT | GCTGGGTCCCGCTTGGCAGCTCG | −117 to −111 |
KBS-4 MUT | GCTGGGTCCCGCTGGGCAGCTCG |
Abbreviations: KBS, Kaiso-binding sequence; MUT, mutated; TGFβR1, transforming growth factor β receptor 1; WT, wild type. KBS—emboldened; CpG dinucleotides—underline; mutated nucleotides—italic.