Table 2. Oligonucleotides representing different potential Kaiso-binding sites in the TGFβR2 promoter.
TGFβR2 probe name | Oligonucleotide sequence (5′–3′) | Location |
---|---|---|
KBS-1 WT | ATGGGCTGGTGGCAGAAGAGGGA | −1401 to −1395 |
KBS-1 MUT | ATGGGCTGGTGGAAGAAGAGGGA | |
KBS-2 WT | CCCTTGCCTCTGCAATCTTCCTC | −1081 to −1075 |
KBS-2 MUT | CCCTTGCCTCTTCAATCTTCCTCT | |
KBS-3 WT | TTACAGTTTCTGCTATACTCTATA | −707 to −701 |
KBS-3 MUT | TTACAGTTTCTGATATACTCTATA | |
KBS-4 WT | AAACATGATTGGCAGCTACGAGA | −35 to −29 |
KBS-4 MUT | AAACATGATTGGAAGCTACGAGA |
Abbreviations: KBS, Kaiso-binding sequence; MUT, mutated; TGFβR2, transforming growth factor β receptor 2; WT, wild type. KBS—emboldened; mutated nucleotides—italic.