Table 3. List of primer sequences used for RT–PCR analysis with their annealing temperatures.

Target		Sequence (5'–3')	Annealing temperature
Kaiso	Forward	TGCCTATTATAACAGAGTCTTT	55 °C
	Reverse	AGTAGGTGTGATATTTGTTAAAG
E-cadherin	Forward	CACCCTGGCTTTGACGCCGA	63 °C
	Reverse	AAACGGAGGCCTGATGGGGCG
ZO-1	Forward	CGGGAAGTTACGTGGCGAA	60 °C
	Reverse	CTCCATTGCTGTGCTCTTGG
Vimentin	Forward	TACGTGACTACGTCCACCCG	63 °C
	Reverse	ATCTCCTCCTGCAATTTCTCCC
Slug	Forward	AGACCCCCATGCCATTGAAG	63 °C
	Reverse	CTTCTCCCCCGTGTGAGTTC
ZEB-1	Forward	AGAATTCACAGTGGAGAGAAGCC	53 °C
	Reverse	CGTTTCTTGCAGTTTGGGCATT
β-Actin	Forward	CTCTTCCAGCCTTCCTTCCT	55 °C

Abbreviation: RT–PCR, reverse transcription–PCR.