Table 1.

Relative lipid contents of strains with overexpressed or deleted genetic targets in addition to DGA1 overexpression

Genetic background		Function	(Fl/OD)mutant/(Fl/OD)NS125
WT		Wild-type Yarrowia lipolytica strain	0.55 ± 0.02
DGA1 (NS125)		Diacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor	1.00 ± 0.09
DGA1	Δgut2	Mitochondrial glycerol-3-phosphate dehydrogenase	0.95 ± 0.12
DGA1	GPD1	NAD-dependent glycerol-3-phosphate dehydrogenase	1.12 ± 0.03
DGA1	ME	Mitochondrial malic enzyme; catalyzes the oxidative decarboxylation of malate to pyruvate	0.92 ± 0.15
DGA1	ACL1/2	ATP citrate lyase 1 & 2	0.99 ± 0.09
DGA1	ACC1	Acetyl-CoA carboxylase subunit 1	1.05 ± 0.19
DGA1	SCT1	Bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase	1.03 ± 0.01
DGA1	SLC1	1-acylglycerol-3-phosphate O-acyltransferase	1.25 ± 0.07
DGA1	SCD1	Stearoyl-CoA desaturase. Endoplasmic reticulum (ER) protein that catalyzes the Δ9-cis desaturation of saturated fatty acids	0.85 ± 0.18
DGA1	DGA2	DGAT1 acyl-CoA:diacylglycerol acyltransferase family	1.62 ± 0.03
DGA1	LRO1	Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis	1.33 ± 0.07
DGA1	Δtgl3	Bifunctional triacylglycerol lipase. Major lipid particle-localized triacylglycerol (TAG) lipase	0.79 ± 0.24
DGA1	Δtgl4	Multifunctional lipase/hydrolase/phospholipase; triacylglycerol lipase, steryl ester hydrolase, and Ca2 + -independent phospholipase A2	1.03 ± 0.08
DGA1	Δmfe1	Multifunctional enzyme, member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase family	0.89 ± 0.03

Lipid content was measured by fluorescence-based lipid assay after 96 h of fermentation in 48-well plates and normalized by the value corresponding to the parental strain NS125 overexpressing DGA1 alone. All genes described here were amplified from Y. lipolytica genomic DNA and sequences are given in Additional file 1