Table 1.
Genetic background | Function | (Fl/OD)mutant/(Fl/OD)NS125 | |
---|---|---|---|
WT | Wild-type Yarrowia lipolytica strain | 0.55 ± 0.02 | |
DGA1 (NS125) | Diacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor | 1.00 ± 0.09 | |
DGA1 | Δgut2 | Mitochondrial glycerol-3-phosphate dehydrogenase | 0.95 ± 0.12 |
DGA1 | GPD1 | NAD-dependent glycerol-3-phosphate dehydrogenase | 1.12 ± 0.03 |
DGA1 | ME | Mitochondrial malic enzyme; catalyzes the oxidative decarboxylation of malate to pyruvate | 0.92 ± 0.15 |
DGA1 | ACL1/2 | ATP citrate lyase 1 & 2 | 0.99 ± 0.09 |
DGA1 | ACC1 | Acetyl-CoA carboxylase subunit 1 | 1.05 ± 0.19 |
DGA1 | SCT1 | Bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase | 1.03 ± 0.01 |
DGA1 | SLC1 | 1-acylglycerol-3-phosphate O-acyltransferase | 1.25 ± 0.07 |
DGA1 | SCD1 | Stearoyl-CoA desaturase. Endoplasmic reticulum (ER) protein that catalyzes the Δ9-cis desaturation of saturated fatty acids | 0.85 ± 0.18 |
DGA1 | DGA2 | DGAT1 acyl-CoA:diacylglycerol acyltransferase family | 1.62 ± 0.03 |
DGA1 | LRO1 | Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that contribute to triglyceride synthesis | 1.33 ± 0.07 |
DGA1 | Δtgl3 | Bifunctional triacylglycerol lipase. Major lipid particle-localized triacylglycerol (TAG) lipase | 0.79 ± 0.24 |
DGA1 | Δtgl4 | Multifunctional lipase/hydrolase/phospholipase; triacylglycerol lipase, steryl ester hydrolase, and Ca2 + -independent phospholipase A2 | 1.03 ± 0.08 |
DGA1 | Δmfe1 | Multifunctional enzyme, member of the peroxisomal hydroxyacyl coenzyme A dehydrogenase family | 0.89 ± 0.03 |
Lipid content was measured by fluorescence-based lipid assay after 96 h of fermentation in 48-well plates and normalized by the value corresponding to the parental strain NS125 overexpressing DGA1 alone. All genes described here were amplified from Y. lipolytica genomic DNA and sequences are given in Additional file 1