Table 4.
Recently reported literature values for lipid titer, yield, and productivity with engineered Yarrowia lipolytica strains
| Parental strain | Strain | Lipid pathway genetic engineering | Process | Sugar consumed (g/L) | Lipid titer (g/L) | Overall yield (g/g) | Overall volumetric productivity (g/L/h) | Cell specific productivity (g/g/h) | Reference |
|---|---|---|---|---|---|---|---|---|---|
| po1g | MTYL037 | control strain (ura + lacZ) | Batch | 80 | 2.5 | 0.03 | 0.02 | 0.002 | [19] |
| po1g | MTYL065 | DGA1 ACC1 | Batch | 80 | 17.6 | 0.20 | 0.14 | 0.017 | [19] |
| po1g | YL-ad9 | DGA1 ACC1 SGD1 | Fed-batch | 235 | 55 | 0.23 | 0.71 | 0.038 | [22] |
| po1f | po1f leu + ura + | control strain | Batch | 80 | 3 | 0.04 | 0.02 | 0.001 | [21] |
| po1f | pex10 mfe1 leu + ura + DGA1 | DGA1 Δpex10 Δmfe1 | Batch | 80 | 16.1 | 0.20 | 0.11 | 0.017 | [21] |
| po1f | pex10 mfe1 leu + ura + DGA1 | DGA1 Δpex10 Δmfe1 | Batch | 160 | 25.3 | 0.16 | 0.21 | – | [21] |
| po1f | L36 DGA1 leu + ura + | DGA1 mga2-G643R | Fed-batch | 160 | 25 | 0.21 | 0.13 | 0.018 | [24] |
| po1f | E26E1 | DGA1 Δpex10 Δmfe1 uga-P209S | Batch | 160 | 39.1 | 0.24 | 0.56 | 0.035 | [23] |
| po1d | JMY2593 | control strain (SUC2) | Batch | 60 | 2.6 | 0.04 | 0.04 | 0.004 | [26] |
| po1d | JMY3582 | DGA2(x3) Δdga1 Δdga2 Δlro1 Δare1 | Batch | 60 | 6.5 | 0.11 | 0.09 | 0.010 | [26] |
| NS18 | NS18 | wild-type | Batch | 60 | 3.6 | 0.06 | 0.04 | 0.002 | this study |
| NS18 | NS432 | DGA1 DGA2 Δtgl3 | Batch | 140 | 30.8 | 0.21 | 0.27 | 0.048 | this study |
| NS18 | NS432 | DGA1 DGA2 Δtgl3 | Fed-batch | 430 | 84.5 | 0.20 | 0.73 | 0.044 | this study |