Figure 1.

Induction of RBOHB by INF1 and R3a/AVR3a.

(A) Expression of RBOHB in response to flg22 and INF1 proteins. INF1 (100 nM) and 100 nM flg22 were infiltrated into leaves by a needleless syringe, and total RNAs were used for RT-qPCR.

(B) Expression of RBOHB in response to MEK2^DD, R3a/AVR3a, and INF1. Total RNAs were extracted from leaves at the indicated hours after agroinfiltration and were used for RT-qPCR.

(C) Effects of single or multiple MAPK gene silencing on RBOHB expression induced by INF1 and R3a/AVR3a. Total RNAs were extracted from SIPK (S)- or NTF6 (N6)-silenced leaves 0, 36, and 48 h after agroinfiltration and were used for RT-qPCR.

Asterisks indicate statistically significant differences compared with water (A), GUS (B), or TRV (C) (t test, *P < 0.05 and **P < 0.01). Data are means ± sd from at least three experiments.