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. 2015 Sep 15;27(9):2645–2663. doi: 10.1105/tpc.15.00213

Figure 3.

Figure 3.

Promoter Activity of RBOHB Induced by INF1 and R3a/AVR3a via the cis-Element.

(A) Deletion analysis of the RBOHB promoter in response to flg22, INF1, and R3a/AVR3a. Leaves were treated with 100 nM INF1 and 100 nM flg22 or were inoculated with Agrobacterium carrying INF1 and R3a/AVR3a. Promoter activities were analyzed as described in Figure 2A.

(B) Analysis of the RBOHB −1000 and mB4-1000 promoter in response to INF1 and R3a/AVR3a.

(C) Activation of three tandem repeats of the cis-element by INF1 and R3a/AVR3a. Promoter activity was represented as a relative value, using the comparative 3 × cis of INF1.

(D) Analysis of INF1- or R3a/AVR3a-induced three-tandem promoter activities in single or multiple MAPK gene-silenced leaves.

(E) Analysis of INF1- or R3a/AVR3a-induced RBOHB −2000 promoter activities in single or multiple MAPK gene-silenced leaves.

Asterisks indicate statistically significant differences compared with water and empty (Emp) (A), mB4-1000 (B), 35S minimal (C), or TRV ([D] and [E]) (t test, *P < 0.05 and **P < 0.01). Data are means ± sd from at least three experiments.