Figure 5.

Regulation of RBOHB Expression by Multiple WRKY Transcription Factors.

(A) Induction of the RBOHB gene by phospho-mimicking mutants of SP cluster-carrying WRKYs. Total RNAs were extracted from leaves 24 h after agroinfiltration and were used for RT-qPCR. nD indicates phospho-mimicking mutants of WRKYs.

(B) Involvement of multiple WRKY genes in MEK2^DD-induced RBOHB expression. Total RNAs were extracted from the WRKY-silenced leaves 0 and 36 h after agroinfiltration and were used for RT-qPCR.

(C) Suppression of INF1- and R3a/AVR3a-induced RBOHB expression by silencing of multiple WRKY genes. Total RNAs were extracted from leaves 0 or 36 h after agroinfiltration and were used for RT-qPCR.

(D) Expression of RBOHB in response to variants of WRKY8 and WRKY11. Total RNAs were extracted from leaves 24 h after agroinfiltration and were used for RT-qPCR. Anti-HA antibody was used to detect accumulations of WRKY-HA variants. nD and nA indicate that WRKY mutants mimic the phosphorylated form and nonphosphorylated form, respectively.

Letters represent each significance group, determined through Tukey's multiple range test. Asterisks indicate statistically significant differences compared with TRV ([B] and [C]) (t test, *P < 0.05 and **P < 0.01). Data are means ± sd from at least three independent experiments.